KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Brilliant Violet 785™ anti-mouse CD45 (30-F11) | Biolegend | Cat#103149; RRID:AB_2564590 |
| Biotin anti-mouse CD3ε (145-2C11) | Biolegend | Cat#100304; RRID:AB_312669 |
| FITC anti-mouse TCRβ (H57-597) | Biolegend | Cat#109206; RRID:AB_313429 |
| PE/Cyanine7 anti-mouse TCRβ (H57-597) | Biolegend | Cat#109222; RRID:AB_893625 |
| APC/Fire™ 750 anti-mouse TCRγ/δ (GL3) | Biolegend | Cat#118136; RRID:AB_2650828 |
| PE anti-mouse TCRγ/δ (GL3) | Biolegend | Cat#118108; RRID:AB_313832 |
| Brilliant Violet 510™ anti-mouse CD4 (GK1.5) | Biolegend | Cat#100449; RRID:AB_2564587 |
| Brilliant Violet 711™ anti-mouse CD8α (53-6.7) | Biolegend | Cat#100747; RRID:AB_11219594 |
| PerCP/Cyanine5.5 anti-mouse CD44 (IM7) | Biolegend | Cat#103032; RRID:AB_2076204 |
| APC anti-mouse PD-1 (29F.1A12) | Biolegend | Cat#135210; RRID:AB_2159183 |
| Brilliant Violet 421™ anti-mouse PD-1 (29F.1A12) | Biolegend | Cat#135221; RRID:AB_2562568 |
| PE anti-mouse CD69 (H1.2F3) | Biolegend | Cat#104507; RRID:AB_313110 |
| PE anti-mouse CD27 (LG.3A10) | Biolegend | Cat#124209; RRID:AB_1236464 |
| APC anti-mouse CCR6 (29-2L17) | Biolegend | Cat#129813; RRID:AB_1877148 |
| PE anti-mouse Vγ1/2 (4B2.9) | Biolegend | Cat#142703; RRID:AB_10960739 |
| APC ant-mouse Vγ4 (UC3-10A6) | Biolegend | Cat#137707; RRID:AB_10899574 |
| PE/Cyanine7 anti-mouse IFNγ (XMG1.2) | Biolegend | Cat#505826; RRID:AB_2295770 |
| PE anti-mouse IL-17A (TC11-18H10.1) | Biolegend | Cat#506904; RRID:AB_315464 |
| TruStain FcX™ PLUS (anti-mouse CD16/32) (S17011E) | Biolegend | Cat#156604; RRID:AB_2783138 |
| FITC CD45 (30-F11), eBioscience | Invitrogen | Cat#11-0451-81; RRID:AB_465049 |
| PE anti-mouse RORγt (B2D), eBioscience | Invitrogen | Cat#12-6981-82; RRID:AB_10807092 |
| PE anti-mouse Nur77 (12.14), eBioscience | Invitrogen | Cat#12-5965-80; RRID:AB_1257210 |
| InVivoMAb™ anti-mouse PD-1 (RMP1-14) | Bio X cell | Cat#BE0146; RRID:AB_10949053 |
| InVivoMAb™ rat IgG2a isotype control (2A3) | Bio X cell | Cat#BE0089; RRID:AB_1107769 |
| InVivoMAb™ anti-mouse/rat IL-1β (B122) | Bio X cell | Cat#BE0246; RRID:AB_2687727 |
| InVivoMAb™ Polyclonal Armenian hamster IgG | Bio X cell | Cat#BE0091; RRID:AB_1107773 |
|
Chemicals, peptides, and recombinant proteins | ||
| LIBERASE™ research grade | Sigma Aldrich | Cat#5401127001 |
| DNase I recombinant, RNase-free solution | Sigma Aldrich | Cat#04716728001 |
| Ampicillin | Duke University Hospital pharmacy | N/A |
| Vancomycin Hydrocholoride | Duke University Hospital pharmacy | N/A |
| Neomycin | Duke University Hospital pharmacy | N/A |
| Metronidazole | Duke University Hospital pharmacy | N/A |
| Fluconazole | Duke University Hospital pharmacy | N/A |
| Dextran sulfate sodium salt (DSS), Mw ~ 40000 | Chem-Impex | Cat#01288 |
| IC fixation buffer | Invitrogen | Cat#00-8222-49 |
| eBioscience™ Fixation/Permeabilization concentrate | Invitrogen | Cat#00-5123-43 |
| eBioscience™ Fixation/Permeabilization diluent | Invitrogen | Cat#00-5223-56 |
| Permeabilization buffer 10× | Invitrogen | Cat#00-8333-56 |
| TRIzol Reagent | Invitrogen | Cat#15596026 |
| Phorbol 12-myristate 13-acetate (PMA) | Sigma Aldrich | Cat#P8139 |
| Ionomycin calcium salt from Streptomyces conglobatus | Sigma Aldrich | Cat#I0634 |
| BD Golgi Plug™ protein transport inhibitor | BD Biosciences | Cat#555029 |
| eBioscience™ RBC Lysis buffer (multi-species) 10× | Invitrogen | Cat#00-4300-54 |
| LIVE/DEAD™ fixable blue dead cell stain kit | Invitrogen | Cat#L34962 |
| LIVE/DEAD™ fixable green dead cell stain kit | Invitrogen | Cat#L23101 |
| MitoTracker™ Green FM | Invitrogen | Cat#M7514 |
| Tetramethylrhodamine, Ethyl Ester, Perchlorate (TMRE) | Invitrogen | Cat#T669 |
| BODIPY™ FL C16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid) | Invitrogen | Cat#D3821 |
|
Critical commercial assays | ||
| TaqMan probe: Il17a | Thermo Fisher Scientific | Mm00439118_m1 |
| TaqMan probe: Il17f | Thermo Fisher Scientific | Mm0052143_m1 |
| TaqMan probe: IFNγ | Thermo Fisher Scientific | Mm01168134_m1 |
| TaqMan probe: Hprt | Thermo Fisher Scientific | Mm03024075_m1 |
| QuantiTect Reverse Transcription kit | Qiagen | Cat#205313 |
| TaqMan™ Gene Expression Master Mix | appliedbiosystems | Cat#4369016 |
|
Experimental models: Organisms/strains | ||
| Mouse: Il17a-GFP mice (C57BL/6-Il17atm1Bcgen/J) | Jackson Laboratory | Strain#018472; RRID:IMSR_JAX:018472 |
| Mouse: Nur77-GFP mice (C57BL/6-Tg (Nr4a1-EGFP/cre)820Khog/J) | Jackson Laboratory | Strain#016617; RRID:IMSR_JAX:016617 |
| Mouse: C57BL/6J | Jackson Laboratory | Strain#000664; RRID:IMSR_JAX:000664 |
| Mouse: Muc2−/− mice | Velcich et al.41 | N/A |
| Germ-free mice | Duke University Gnotobiotic Core | N/A |
|
Software and algorithms | ||
| FlowJo™ v10 | BD Biosciences | https://www.flowjo.com/solutions/flowjo/downloads |
| GraphPad Prism v9 | GraphPad | https://www.graphpad.com/ |
|
Other | ||
| BD LSRFortessa X20 Cell Analyzer | BD Biosciences | N/A |
| BD LSRFortessa Cell Analyzer | BD Biosciences | N/A |
| GentleMACS™ Dissociator | Miltenyi Biotec Inc. | N/A |