Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Oct 20;220(12):e20230183. doi: 10.1084/jem.20230183

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Titcombe et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S2. — BATF drives expression of PD-1 but is dispensable for FR4 and NRP1 upregulation during tolerance induction. (A and B) Naïve KRN T cells were modified by CRISPR-Cas9 in the presence or absence of Batf gRNA (as indicated) and then subjected to tolerization for 7 days by adoptive transfer into B6.I-A^b/g7 hosts. Shown are the day 7 FR4 × PD-1 (A) and NRP1 × PD-1 (B) flow cytometry profiles for tolerized KRN T cells. Normal host polyclonal CD44^hi CD4⁺ T cells are also displayed for reference. Mice as in Fig. 3 D. (C and D) KRN T cells were modified by CRISPR-Cas9 in the presence of Thy1, Batf, or Batf plus Bcl2l11 gRNAs and then subjected to tolerization for 7 days. (C) Expression of Thy1, BATF, and Ki67 in modified T cells. (D) PD-1 expression in tolerized KRN T cells with representative flow plots shown (left) and conventional PD-1⁺ KRN T cell numbers quantitated (right). Unpaired t test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s. = not significant. Source data as in Fig. 6.