Table 1. Primers used for real-time quantitative PCR.
Gene | Forward primer (5´ - 3´) | Reverse primer (5´ - 3´) |
---|---|---|
Ppar α | TGGGGATGAAGAGGGCTGAG | GGGGACTGCCGTTGTCTGT |
Ppar γ | TGTCGGTTTCAGAAGTGCCTTG | TTCAGCTGGTCGATATCACTGGAG |
Srebp-1c | CGGCGCGGAAGCTGT | AGTCACTGTCTTGGTTGTTGATGAG |
Fas | ATCCTGGAACGAGAACACGATCT | AGAGACGTGTCACTCCTGGACTT |
Tnfα | AAGCCTGTAGCCCACGTCGTA | GGCACCACTAGTTGGTTGTCTTG |
IL-1β | TCCAGGATGAGGACATGAGCAC | GAACGTCACACACCAGCAGGTTA |
Tlr-2 | CCCTTCTCCTGTTGATCTTGCT | CGCCCACATCATTCTCAGGTA |
Tlr-4 | GCAGCAGGTGGAATTGTATCG | TGTGCCTCCCCAGAGGATT |
Tlr-6 | AACCTTACTCATGTCCCCAAAGAC | GCATCCGAAGCTCAGATATAGAGTT |
Tlr-9 | CCTTGACAACCTCCCCAAGA | AGGACTTCCAGGTTGGGTAGGA |
αSma | ACCAACTGGGACGACATGGAA | TGTCAGCAGTGTCGGATGCTC |
Tgf-β1 | GTGTGGAGCAACATGTGGAACTCTA | TTGGTTCAGCCACTGCCGTA |
Col1a1 | GCACGAGTCACACCGGAACT | AAGGGAGCCACATCGATGAT |