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. 2023 Oct 20;12:RP88523. doi: 10.7554/eLife.88523

Figure 4. Effects of C-type natriuretic peptide (CNP) on the fertilization ability and embryonic development of aged oocytes.

(A) Representative images of early embryos developed from young, aged, and aged + CNP oocytes in vitro fertilization. Scale bar, 100 μm. (B) The fertilization rate (two-cell embryos rate), (C) four-cell embryos rate, (D) eight-cell embryos rate, (E) morula rate, and (F) blastocyst formation rates were recorded in the young, aged, and aged + CNP groups. Data in (B–F) are presented as mean percentage (mean ± SEM) of at least three independent experiments.

Figure 4.

Figure 4—figure supplement 1. Effects of C-type natriuretic peptide (CNP) on the fertilization ability and embryonic development in vitro maturation oocytes.

Figure 4—figure supplement 1.

Data are presented as mean percentage (mean ± SEM) of three independent experiments. *Significant difference (p<0.05), **significant difference (p<0.01).