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. 2023 Oct 20;12:RP88523. doi: 10.7554/eLife.88523

Figure 5. Effect of C-type natriuretic peptide (CNP) supplementation on transcriptome profiling of aged oocytes.

(A) Heatmap illustration displaying gene expression of young, aged, and aged + CNP oocytes. (B) Volcano plot showing differentially expressed genes (DEGs; downregulated, blue; upregulated, red) in young vs. aged oocytes. Some highly DEGs are listed. (C) Volcano plot showing DEGs in aged vs. aged + CNP oocytes. Some highly DEGs are listed. (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of upregulated and downregulated DEGs in young vs. aged oocytes. (E) KEGG enrichment analysis of upregulated and downregulated DEGs in aged vs. aged + CNP oocytes.

Figure 5—source data 1. RNAseq dataset.

Figure 5.

Figure 5—figure supplement 1. The relative expression of several randomly selected genes from each group was verified using quantitative real-time PCR.

Figure 5—figure supplement 1.

(A) RNA-seq results of selected genes in oocyte from young, aged, and aged + C-type natriuretic peptide (CNP) mouse. (B) The relative expression of the randomly selected genes was verified by RT-qPCR in oocyte from young, aged, and aged + CNP mouse.