Figure 5. The RGD motifs play a role in the structural integrity of PrgB.

(A) Close-up view of the RGD motif in the CSA1 domain (purple). Most of the important interactions, mainly hydrogen bonds, formed by this motif are to residues on the CSC1 domain (green). (B) Close-up view of the RGD motif in the CSA2 domain (sand colored). Most of the important interactions, mainly hydrogen bonds, formed by this motif are to residues on the CSC2 domain (dark red). Potential hydrogen bonds are marked by lines and the surface of the protein indicated by transparent gray.

Figure 5—figure supplement 1. Schematic overview of previously described prgB mutants.

(A) Red arrows indicate the sites of transposon insertions that cause defective phenotypes in aggregation, biotic tissue biofilm formation, or conjugation. (B) Diagrams illustrating the specific deletion mutants discussed in the main text. Dashed lines indicate deleted regions. SS, signal sequence; UNS, unstructured region; PAD, polymer adhesin domain; COI, coiled-coil domain; CSA, cell-surface antigen; CSC, cell-surface antigen C-terminus; CW, LPXTG-containing cell wall anchor sequence.