Figure	Genotype
Figure 1D–F	w/w, 20xUAS-CsChrimson-mVenus attP18;;+/Gr64f-split-GAL4 (SS87269)
Figure 1H–L Figure 1—figure supplement 1	w/w, 20xUAS-CsChrimson-mVenus attP18;;+/MB043C-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;+/MB213B-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;;+/MB312C-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;MB109B/MB315C-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;+/ Empty-split-GAL4
Figure 2 Figure 2—figure supplements 1 and 2	w/w, 20xUAS-CsChrimson-mVenus attP18;Split-GAL4/+
Figure 3A	w/w, 20xUAS-CsChrimson-mVenus attP18;SS33917-Split-GAL4/+
Figure 3—figure supplement 2	pBPhsFlp2::PEST in attP3;; pJFRC201-10XUAS-FRT>STOP > FRT-myr::smGFP-HA in VK0005, pJFRC240-10XUAS- FRT>STOP > FRT-myr::smGFP-V5-THS-10XUAS-FRT>STOP > FRT-myr::smGFP-FLAG in su(Hw)attP1/SS33917-split-GAL4
Figure 4A Figure 4B Figure 4C	LexAop2-Syn21-opGCaMP6s (JK16F), LexAop2-Syn21-opGCaMP6s (SuHwattP8), 10XUAS-Syn21-Chrimson88-tdT-3.1 (attP18)/+; R64A11-LexAp65 (JK73A)/MB082C LexAop2-Syn21-opGCaMP6s (JK16F), LexAop2-Syn21-opGCaMP6s (SuHwattP8), 10XUAS-Syn21-Chrimson88-tdT-3.1 (attP18)/+; R64A11-LexAp65 (JK73A)/MB310C LexAop2-Syn21-opGCaMP6s (JK16F), LexAop2-Syn21-opGCaMP6s (SuHwattp8), 10xUAS-Syn21-Chrimson88-tdT-3.1 (attp18); G0239-GAL4/G0239-GAL4; R64A11-LexAp65 (JK73A)/+ LexAop2-Syn21-opGCaMP6s (JK16F), LexAop2-Syn21-opGCaMP6s (SuHwattp8), 10xUAS-Syn21-Chrimson88-tdT-3.1 (attp18); G0239-GAL4/G0239-GAL4; R64A11-LexAp65 (JK73A)/MB310C LexAop2-Syn21-opGCaMP6s (JK16F), LexAop2-Syn21-opGCaMP6s (SuHwattp8), 10xUAS-Syn21-Chrimson88-tdT-3.1 (attp18); G0239-GAL4/+; R64A11-LexAp65 (JK73A)/MB310C
Figure 4—figure supplement 1	LexAop2-Syn21-opGCaMP6s in su(Hw)attP8,10XUAS-Syn21-Chrimson88-tdT-3.1 in attP18; 10XUAS-LexAp65-DBD2-RNAi (VK2)/; 64A11-LexAp65 JK73A/ SS67249
Figure 5	13XLexAop2-IVS-p10-ChrimsonR-mVenus (attP18); 58E02-LexAp65 (attP40)/ss67249-split1; pJFRC28-10XUAS-IVS-GFP-p10 (SuHwattP1) / ss67249-split2
Figure 5—figure supplement 1	pBPhsFlp2::PEST in attP3;; pJFRC201-10XUAS-FRT>STOP > FRT-myr::smGFP-HA in VK0005, pJFRC240-10XUAS- FRT>STOP > FRT-myr::smGFP-V5-THS-10XUAS-FRT>STOP > FRT-myr::smGFP-FLAG in su(Hw)attP1/SS67249-split-GAL4
Figure 5—figure supplement 2	13XLexAop2-IVS-p10-ChrimsonR-mVenus (attP18); 58E02-LexAp65 (attP40)/ss67249-split1; pJFRC28-10XUAS-IVS-GFP-p10 (SuHwattP1) / ss67249-split2
Figure 6	w/w, 20xUAS-CsChrimson-mVenus attP18;SS33917-Split-GAL4/+w/w, 20xUAS-CsChrimson-mVenus attP18;SS33918-Split-GAL4/+w/w, 20xUAS-CsChrimson-mVenus attP18;MB077B-Split-GAL4/+w/w, 20xUAS-CsChrimson-mVenus attP18;Empty-Split-GAL4/+
Figure 6—figure supplement 1	w/w, 20xUAS-CsChrimson-mVenus attP18;Split-GAL4/+
Figure 7A–B	w/+;SS33917-split-GAL4/+w/+; SS33917-split-GAL4UAS-TNT (II) w/+; Empty-split-GAL4UAS-TNT (II)
Figure 7C	w/w;VT007746-p65ADZp in attP40/20xUAS-Shbire-p10 in VK00005 w/w;R64A11-ZpGAL4DBD in attP2/20xUAS-Shbire-p10 in VK00005 w/w;SS33917(VT007746-p65ADZp in attP40; R64A11-ZpGAL4DBD in attP2)/20xUAS-Shbire-p10 in VK00005 w/w;Empty-split-GAL4/20xUAS-Shbire-p10 in VK00005
Figure 7D	w/w, 20xUAS-CsChrimson-mVenus attP18;SS33917/+w/w, 20xUAS-CsChrimson-mVenus attP18;Empty-split-GAL4 /+
Figure 7E	w/w, 20xUAS-CsChrimson-mVenus attP18;SS33917/+w/w, 20xUAS-CsChrimson-mVenus attP18;SS33918/+w/w, 20xUAS-CsChrimson-mVenus attP18;SS49755/+w/w, 20xUAS-CsChrimson-mVenus attP18;Empty-split-GAL4 /+
Figure 7—figure supplement 1	w/w, 20xUAS-CsChrimson-mVenus attP18;SS33917/+w/w, 20xUAS-CsChrimson-mVenus attP18;SS33918/+w/w, 20xUAS-CsChrimson-mVenus attP18;SS49755/+w/w, 20xUAS-CsChrimson-mVenus attP18;Gr64f-GAL4;Gr64f-GAL4/+w/w, 20xUAS-CsChrimson-mVenus attP18;Empty-split-GAL4 /+