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. 2023 Sep 28;12:e86084. doi: 10.7554/eLife.86084

Figure 1. Presynaptic Spinophilin functions to sustain homeostatic plasticity through active zone remodeling.

(a,c) Representative images of third-instar larval muscle 4 NMJs immunostained with antibodies against BRP and Unc13A. Scale bars: 2 µm. (a,d) Control animals show an increase in BRP and Unc13A levels upon PhTx treatment while Spn mutants do not (b,d) mean BRP and Unc13A intensities measured through confocal imaging. (e,f) Representative traces of eEJP and mEJP measurements at third-instar larval muscle 6/7 NMJs. Scale bars: eEJP, 10 ms, 10 mV; mEJP traces 500 ms, 5 mV. (g,j) mEJP amplitudes are reduced upon PhTx treatment. eEJP amplitudes are compensated (h) and QC is increased (i) in control and Spn mutants upon 10 min PhTx treatment. eEJP amplitudes are compensated (k) and QC is increased (l) in control but not Spn mutants upon 30 min PhTx treatment. Also see Figure 1—figure supplements 1 and 2. Source data as exact normalized and raw values, detailed statistics including sample sizes and P values are provided in Figure 1—source data 1. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; n.s., not significant, p > 0.05. All panels show mean ± s.e.m.

Figure 1—source data 1. Table containing exact values for the data depicted in Figure 1, along with details of statistical analyses.

Figure 1.

Figure 1—figure supplement 1. Nrx-1, Nlg1 and Nlg2 mutants undergo homeostatic plasticity, while Spn mutants cannot sustain homeostatic plasticity.

Figure 1—figure supplement 1.

(a–h) Wildtype, Nrx-1, Nlg1, and Nlg2 mutants all show normal BRP increase upon PhTx treatment. (i–k) Graphs of raw values corresponding to Figure 1e and g–I (l-n) Graphs of raw values corresponding to Figure 1f and j–l. Source data as exact normalized and raw values, detailed statistics including sample sizes and p values are provided in Figure 1—figure supplement 1—source data 1. *p≤0.05; **p ≤ 0.01; ***p ≤ 0.001; n.s., not significant, p > 0.05. All panels show mean  ± s.e.m.
Figure 1—figure supplement 1—source data 1. Table containing exact values for the data depicted in Figure 1—figure supplement 1, along with details of statistical analyses.
Figure 1—figure supplement 2. Presynaptic Spinophilin is sufficient to sustain homeostatic plasticity.

Figure 1—figure supplement 2.

(c) Representative traces of mEJP and eEJP measurements at third-instar larval muscle 6/7 NMJs. Scale bars: eEJP, 10ms, 10 mV; mEJP traces 500ms, 5 mV. (d,g,j,m) mEPSP amplitudes are lower upon PhTx treatment. (e–f, h–i) Presynaptic motoneuronal expression of full-length Spn reinstates functional plasticity upon PhTx treatment in Spn mutants. Both normalized and raw values have been graphed. (k–l, n–o) Postsynaptic muscle expression of full-length Spn results in moderate functional plasticity upon PhTx treatment in Spn mutants. Both normalized and raw values have been graphed. Source data as exact normalized and raw values, detailed statistics including sample sizes and p values are provided in Figure 1—figure supplement 2—source data 1. *p≤0.05; **p ≤ 0.01; ***p ≤ 0.001; n.s., not significant, p > 0.05. All panels show mean  ± s.e.m.
Figure 1—figure supplement 2—source data 1. Table containing exact values for the data depicted in Figure 1—figure supplement 2, along with details of statistical analyses.