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. 2023 Sep 28;12:e86084. doi: 10.7554/eLife.86084

Figure 2. Spinophilin/Syd-1 antagonism controls active zone structural remodeling and functional plasticity.

(a,c,m) Representative images of third-instar larval muscle 4 NMJs immunostained with an antibody against BRP. Scale bars: 2 µm. (a–b) Control and Syd-1 mutant animals show an increase in BRP upon 10 min PhTx treatment (c–d) Removing a single syd-1 gene copy from Spn mutants (Spn,Syd-1/+) reinstates the BRP increase upon 10 min PhTx treatment (b,d) mean BRP intensities measured through confocal imaging. (e,f) Representative traces of mEJP and eEJP measurements at third-instar larval muscle 6/7 NMJs. Scale bars: eEJP, 10 ms, 10 mV; mEJP traces 500 ms, 5 mV. (g,j) mEJP amplitudes are reduced upon PhTx treatment. eEJP amplitudes are compensated (h) and QC is increased (i) in control and Syd-1 mutants upon 30 min PhTx treatment. eEJP amplitudes are compensated (k) and QC is increased (l) in control and single copy syd-1 in Spn (Spn,Syd-1/+) mutants upon 30 min PhTx treatment. (m–n) Syd-1 mutant animals continue to show an increase in BRP upon 30 min PhTx treatment, while controls do not. Also see Figure 2—figure supplement 1. Source data as exact normalized and raw values, detailed statistics including sample sizes and P values are provided in Figure 2—source data 1. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; n.s., not significant, p > 0.05. All panels show mean ± s.e.m.

Figure 2—source data 1. Table containing exact values for the data depicted in Figure 2, along with details of statistical analyses.

Figure 2.

Figure 2—figure supplement 1. Spinophilin/Syd-1 antagonism sustains homeostatic plasticity.

Figure 2—figure supplement 1.

(a–c) Raw values corresponding to Figure 2e and g–i. (d-f) Graphs of raw values corresponding to Figure 2f and j–l. Source data as exact normalized and raw values, detailed statistics including sample sizes and p values are provided in Figure 2—figure supplement 1—source data 1. *p≤0.05; **p ≤ 0.01; ***p ≤ 0.001; n.s., not significant, p > 0.05. All panels show mean  ± s.e.m.
Figure 2—figure supplement 1—source data 1. Table containing exact values for the data depicted in Figure 2—figure supplement 1, along with details of statistical analyses.