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. 2023 Sep 28;12:e86084. doi: 10.7554/eLife.86084

Figure 5. Rescue of Spinophilin plasticity deficits after genetic and pharmacological disruption of F-actin.

(a,g) Representative images of third-instar larval muscle 4 NMJs immunostained with an antibody against BRP. Scale bars: 2 µm. (a–b) motoneuronal expression of full-length Spn and Mical in Spn mutant background reinstates BRP increase upon PhTx treatment, while MicalΔRedox expression does not. (g–h) Latrunculin-B (LatB) treatment results in an increase in BRP in both control and Spn mutants. (B,H) mean BRP intensities measured through confocal imaging. (c,i) Representative traces of mEJP and eEJP measurements at third-instar larval muscle 6/7 NMJs. Scale bars: eEJP, 10ms, 10 mV; mEJP traces 500ms, 5 mV. (d,j) mEJP amplitudes are reduced upon PhTx treatment. eEJP amplitudes are compensated (e) and QC is increased (f) in motoneuronal expression of full-length Spn and Mical in Spn mutant background upon 30 min PhTx treatment but not in MicalΔRedox. eEJP amplitudes are compensated (k) and QC is increased (l) in control animals and Spn mutants treated with LatB. Also see Figure 5—figure supplement 1. Source data as exact normalized and raw values, detailed statistics including sample sizes and p values are provided in Figure 5—source data 1. *p≤0.05; **p ≤ 0.01; ***p ≤ 0.001; n.s., not significant, p > 0.05. All panels show mean  ± s.e.m.

Figure 5—source data 1. Table containing exact values for the data depicted in Figure 5, along with details of statistical analyses.

Figure 5.

Figure 5—figure supplement 1. Actin depolymerization via Mical could underlie Spinophilin-mediated homeostatic plasticity.

Figure 5—figure supplement 1.

(a–b) Spn mutants show a reduction in Mical levels at the NMJ compared to controls, while Syd-1 mutants show levels comparable to controls. (c–d) Spn mutants show a reduction in Mical levels at the NMJ compared to controls, while Spn mutants with syd-1 heterozygosity show levels comparable to controls. (e–g) Graphs of raw values corresponding to Figure 5c–f. (h–j) Graphs of raw values corresponding to Figure 5i–l. Source data as exact normalized and raw values, detailed statistics including sample sizes and p values are provided in Figure 5—figure supplement 1—source data 1. *p≤0.05; **p ≤ 0.01; ***p ≤ 0.001; n.s., not significant, p > 0.05. All panels show mean  ± s.e.m.
Figure 5—figure supplement 1—source data 1. Table containing exact values for the data depicted in Figure 5—figure supplement 1, along with details of statistical analyses.