TABLE 2.
Oligodeoxyribonucleotides used for amplification
| Primer | Sequence | Location | Strand | Size (bp) of product |
|---|---|---|---|---|
| BL | 5′-TATGAGTGGCTAAATCGAT-3′ | aac(6′)-Ib (28)a | + | 395 |
| BR | 5′-CCCGCTTTCTCGTAGCA-3′ | aac(6′)-Ib (422) | − | |
| QL | 5′-GTCATCGCAACTTCCGCACTG-3′ | qacF (43) | + | 245 |
| QR | 5′-CTGACGATAAGTCCCAT-3′ | qacF (287) | − | |
| IL | 5′-GAGTTGCTGTTCAATGA-3′ | aac(6′)-Ib (331) | + | 948 (IPCR) |
| IR | 5′-TAACTTTGTTTTAGGGC-3′ | 5′ conserved segment | − | |
| L1 | 5′-GGCATCCAAGCAGCAAG-3′ | 5′ conserved segment | + | |
| CL | 5′-ATGGGACTTATCGTCAG-3′ | End of qacF (271) | + | 1,678 |
| CR | 5′-TAACGCTTGAGCTCAGCCGACCGA-3′ | cmlA2 cassette (1345) | − |
a
The positions of the 5′ ends of the internal oligonucleotides are indicated (position numbers are in parentheses) relative to the putative start codon of the corresponding gene.