Fig. 2. CoCl₂ increases nuclear HIF-1α and YAP protein-protein interaction under high cell density.

A MDCK cells at low and high density were incubated with or without 400 μM CoCl₂ for 8 h. The immunoprecipitations were performed and analyzed by Western blotting with anti-YAP antibodies. IgG was used as a loading control. Total cell lysates were subjected to Western blotting with indicated antibodies, and α-tubulin was used as a loading control. B The negative control for the co-immunoprecipitation experiment was performed using an anti-IgG antibody. Whole cell lysate (WCL) is the positive control for anti-YAP and anti-HIF-1α antibodies. IgG was used as a loading control of immunoprecipitation samples. C MDCK cells at low and high density were treated with or without 400 μM CoCl₂ for 8 h. Both nuclear and cytosol extracts were analyzed by Western blotting. Lamin B1 and α-tubulin were used as a loading control for the nuclear and cytosolic proteins.