Fig. 7. Protective effects of YAP under hypoxic conditions.

A, B MDCK-parental, MDCK-shNC, and MDCK-shYAP cells were subjected to normoxia or 1% O₂ for 24 h, followed by immunostaining with anti-γH2AX antibody. Scale bar: 40 μm. γH2AX intensity was analyzed and presented as mean ± SEM in (B). ***p < 0.001, **p < 0.01, ^$$$p < 0.001, and ^###p < 0.001. C, D MDCK-parental, MDCK-shNC, and MDCK-shYAP cells were subjected to normoxia or 1% O₂ for 36 h, followed by Annexin V staining. Scale bar: 20 μm. D Annexin V positive cell number was analyzed and presented as mean ± SEM. ***p < 0.001 vs. 1% O₂ in parental cells, ^$$$p < 0.001 vs. 1% O₂ in shNC cells.