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. 2023 Oct 20;14:6639. doi: 10.1038/s41467-023-42428-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Flow cytometry of cDC in spleen (top row), pLN (middle row) and thymus (bottom row) from WT and Zeb1-dcKO mice. b Frequencies and numbers of XCR1⁺SIRPα^- cDC1 and XCR1^-SIRPα⁺ cDC2 among cDC in spleen, pLN, thymus from mice as in a (spleen: WT, n = 4; Zeb1-dcKO, n = 5; pLN: WT, n = 5; Zeb1-dcKO, n = 4; thymus: WT, n = 3; Zeb1-dcKO, n = 4). c Flow cytometry of cDC in liver (top row) and lung (bottom row) from WT and Zeb1-dcKO mice. d Frequencies and numbers of CD103⁺CD11b⁻ cDC1 and CD103^-CD11b⁺ cDC2 among cDC in liver, lung from mice as in c (WT, n = 3; Zeb1-dcKO, n = 4). e Flow cytometry of Lin^- myeloid cells (Lin^-=CD3^-CD19^- hereinafter) in spleen (top row) and pLN (bottom row) from WT and Zeb1-dcKO mice. f Frequency and number of CD317⁺Ly6C⁺ pDC among Lin^- myeloid cells from mice as in e (spleen, WT, n = 4; Zeb1-dcKO, n = 4; pLN, WT, n = 3; Zeb1-dcKO, n = 4). g UMAP plot showing unsupervised clustering of total splenic cDC (top row) and the population individually (bottom row) from WT and Zeb1-dcKO mice by scRNA-seq analysis. h Flow cytometry of splenic cDC1 (top row) and cDC2 (bottom row) from WT and Zeb1-dcKO mice. i Frequencies of dying (Annexin V⁺ 7-AAD⁺) splenic cDC1 and cDC2 among total cDC1 or cDC2 from mice as in h (WT, n = 3; Zeb1-dcKO, n = 4). j Flow cytometry of splenic cDC from mixed BM chimeric mice. k Frequencies of donor cDC (among total cDC) and cDC1 (among donor cDC) in spleen from mixed BM chimeric mice as in j (B6:WT, n = 8; B6:KO, n = 10). l Flow cytometry of CD11b⁺ cells in spleen of mixed BM chimeric mice. m Frequencies of donor CD11b⁺ cells (among total CD11b⁺ cells) and CD11b⁺Ly6G⁺ neutrophils cells (among donor CD11b⁺ cells) in spleen of mixed BM chimeric mice as in l (B6:WT, n = 4; B6:KO, n = 5). Each symbol represents an individual mouse, small horizontal lines indicate the mean (± s.d.). Data are representative of two (h–m) or three (a–f) independent experiments. Data are presented as mean ± s.d. Statistical analysis was performed using two-tailed unpaired Student’s t-test. Source data are provided as a Source Data file.