Fig. 4. Zeb1 is selectively required for cross-presentation by cDC1.
a Flow cytometry of OT-I T cells from WT and Zeb1-dcKO mice receiving adoptive transfer of 5 × 105 CFSE-labeled OT-I T cells, at day 3 after i.v. immunization of 5 × 105 irradiated ovalbumin (OVA)-loaded β2m−/− splenocytes. b Frequency of CFSE-CD44+ OT-I T cells among total OT-I T cells from mice as in a (WT, n = 5; Zeb1-dcKO, n = 4). Each symbol represents an individual mouse, small horizontal lines indicate the mean (± s.d.). cDC1 sorted from pLNs (c) or mLNs (d) of WT and Zeb1-dcKO mice were cultured for 3 days with CFSE-labeled OT-I T cells and different dose of HKLM-OVA, and assayed for OT-I proliferation and activation (CFSE-CD44+). WT and Zeb1-deficient Flt3L-cDC1 (e) or Flt3L-cDC2 (f) were cultured and analyzed as described in c and d, with different dose of irradiated OVA-loaded β2m-/- splenocytes as antigens. Flt3L-cDC1 (g) or Flt3L-cDC2 (h) of both genotypes were cultured and analyzed as described in c and d, with various dose of HKLM-OVA as antigens. Flt3L-cDC1 (i) or Flt3L-cDC2 (j) of both genotypes were cultured and analyzed as described in c and d, with various dose of soluble OVA as antigens. Flt3L-cDC1 (k) or Flt3L-cDC2 (l) of both genotypes were cultured and analyzed as described in c and d, with different amounts of SIINFEKL peptides as antigens. Data are representative of three independent experiments. Data are presented as mean ± s.d. Statistical analysis was performed using two-tailed unpaired Student’s t-test (b) or two-way ANOVA with Sidak’s multiple comparisons test (c–l). Source data are provided as a Source Data file.