Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Oct 20;14:6639. doi: 10.1038/s41467-023-42428-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Volcano plot illustrating DEGs in WT and Zeb1-deficient Flt3L-cDC1 challenged with HKLM-OVA for 4 h (n = 2). b Bubble plot depicting KEGG pathway analysis of DEGs in WT and Zeb1-deficient Flt3L-cDC1 after stimulation as in a (n = 2). GSEA profiles of KEGG pathways of antigen processing and presentation (c), phagosome (d), lysosome pathway (e), in WT and Zeb1-deficient Flt3L-cDC1 after stimulation as in a. f Heatmaps of differentially expressed signature genes in pathways of antigen processing and presentation, phagosome and lysosome in WT and Zeb1-deficient Flt3L-cDC1 after stimulation as in a (n = 2). g Immunoblot analysis of NOX2 subunits Cybb and Ncf2 in WT and Zeb1-deficient Flt3L-cDC1 after stimulation with HKLM-OVA for 4 h. Relative protein level is quantified by normalization to actin protein (n = 3, from 3 independent experiments). h Bubble plot presenting KEGG pathway analysis of DEGs in intersection of Zeb1-regulated genes obtained from above bulk RNA-seq and Zeb1-bound genes obtained from CUT&Tag with Flt3L-cDC1 after stimulation. i Integrative Genomics Viewer (IGV) showing Zeb1 binding peaks (CUT&Tag) in indicated gene loci of WT Flt3L-cDC1 challenged with HKLM-OVA for 4 h. j miRNA-seq illustrating expression levels of the indicated miRNAs in WT and Zeb1-deficient Flt3L-cDC1 challenged with HKLM-OVA for 4 h (n = 2 biologically independent samples in one experiment). k Relative luciferase activity in HEK293T cells transfected with dual reporter vector containing WT or mutated miR-96/182- or miR-467d-binding sites in Cybb together with empty or miR-96- or miR-182- or miR-467d-expressing vector. Renilla luciferase activity is normalized to firefly luciferase activity. * marked on nucleotides represents mutated sites. Each symbol represents an individual sample, small horizontal lines indicate the mean (±s.d.). Data are representative of three independent experiments (g, k) (error bars, s.d.). Data are presented as mean ± s.d. Statistical analysis was performed using two-tailed unpaired Student’s t-test. Source data are provided as a Source Data file.