A WB analysis of the indicated proteins in PSC (H9 ESC and FBXL4MUT iPSC) and CNs derived from H9 ESC and FBXL4MUT iPSC (on Day 30). PSC: Pluripotent stem cells; ESC: Embryonic stem cells; iPSC: Induced pluripotent stem cells; CNs: cortical neurons. Representative images (B) of DCX, HSP60, BNIP3 and BNIP3L antibody immunostaining in 500 µM DFP treated H9/FBXL4MUT CNs (right) with untreated H9/FBXL4MUT CNs (left). Scar bar, 5 µm. Comparing the HSP60, BNIP3 (or BNIP3L) immunostaining intensity of DCX positive cells (C). Data were shown as means ± SD (n = 50). P values are calculated by the Two-way ANOVA test. **P < 0.005; ****P < 0.0001. Representative live cell imaging (D) of 500 µM DFP treated H9/FBXL4MUT CNs with untreated H9/FBXL4MUT CNs, stained with Mtphagy Dye and Lyso Dye, and quantitative analysis (E) of mitophagy levels. Scar bar, 10 µm. Data were shown as means ± SD (n = 50). P values are calculated by the Two-way ANOVA test. ****P < 0.0001. F WB analysis of the indicated proteins in DFP (500 µM)-treated H9/FBXL4MUT CNs with untreated H9/FBXL4MUT CNs (left) on Day 30.