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. 2023 Oct 20;14:6644. doi: 10.1038/s41467-023-42286-3

Fig. 5. ORTW and RT outcomes in NSCLC xenograft tumor model.

Fig. 5

a CEST and BOLD MR imaging of BALB/c nude mice bearing NCI-H460 lung xenografts after intratumoral injection of oxygenated Gly-PFOBs(O2) or PFOBs(O2) (b) over time. c Dynamic CEST MR signal changes of tumor region after Gly-PFOBs (O2) or PFOBs(O2) injection. *P < 0.05 (P = 0.0428); **P < 0.01 (P = 0.0030); ****P < 0.0001, Gly-PFOBs (O2) vs. PFOBs(O2). Two-way ANOVA with Sidak’s multiple comparisons test. Data are presented as mean ± SD, n = 7 mice for Gly-PFOBs (O2) group and n = 6 mice for PFOBs(O2) group. d Determination of the pH changes in the TME before and after Gly-PFOBs (O2) probe injection after fully oxygenated. ****P < 0.0001. One-way ANOVA with Dunnett’s multiple comparisons test. Data are presented as mean ± standard deviation (SD) (n = 6 biologically independent measurements). e Monitoring of tumor tissue oxygenation by calculating changes in T2*values. *P < 0.05 (P = 0.0291). Two-way ANOVA with Sidak’s multiple comparisons test. Data are presented as mean ± SD (n = 5). f, g Statistical results of relative tumor volume and tumor weight (e) of each treatment group. *P < 0.05 (P = 0.0252, RT vs. control, 14d), **P < 0.01 (P = 0.0060, RT+Gly-PFOBs (O2) 0 h vs. control, 14d), **P < 0.01 (P = 0.0069, RT+Gly-PFOBs (O2) 3 h vs. control, 14d), ****P < 0.0001 (RT+Gly-PFOBs (O2) 1~2 h vs. control, 14d). Two-way ANOVA, Tukey’s multiple comparisons test. *P = 0.0245 for g, RT+Gly-PFOBs (O2) 1~2 h vs. control, 14d, one-way ANOVA, Tukey’s multiple comparisons test. Data are presented as mean ± standard deviation (SD) (n = 4 mice). In the boxplots, the center line, box limits, and whiskers denote the median, upper and lower quartiles, and 1.5 × interquartile range, respectively. h Representative H&E, CD31, Ki67, and TUNEL-related antigen staining in different treatment groups (n = 4), scale bar: 100 μm. ORTW optimized radiotherapy time window, BOLD blood oxygen level-dependent, RT radiotherapy. Source data are provided as a Source data file.