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. 2023 Aug 30;30(10):2265–2279. doi: 10.1038/s41418-023-01217-x

Fig. 4. METTL3-mediated regulation of CD70 is dependent on YTHDF2.

Fig. 4

A Putative m6A modification sites in the CD70 mRNA 3’UTR. B, C The relative luciferase activity of the CD70 3’UTR reporter construct with wild type or synonymous mutant m6A sites in METTL3-knockdown and METTL3-overexpressing PTC and ATC cells are shown. D CD70 mRNA levels, E CD70 protein levels and F half-life of CD70 mRNA after actinomycin D treatment in METTL3-overexpressing K1 and BHT101 cells transfected with si-YTHDF2. G Percentage of CD4+Foxp3+ Tregs, CD8+PD-1+Tim-3+ terminally exhausted T cells, CD8+GzmB+perforin+ T cells, CD8+TNF-α+ T cells and CD8+IFN-γ+ T cells in PBMCs co-cultured in the presence of METTL3-overexpressing K1 and BHT101 cells transfected with si-YTHDF2. Data of are presented as the mean ± SD of three independent experiments. The p-values in (B), (C), (D), (F) and (G) were calculated by Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001.