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. 2023 Oct 20;9:85. doi: 10.1038/s41523-023-00588-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A A pan-cancer analysis between TRIM21 and signaling pathways by Gene Set Cancer Analysis. B Potential E3 ligases or substrates are predicted by using UbiBrowser. C TRIM21-BRCA1 Interaction analysis was performed in the GeneMANIA database. The specific interaction and predictive values are visible on (http://genemania.org/search/homo-sapiens/TRIM21/BRCA1). D The protein level was examined after transfecting with exogenous full-length BRCA1, TRIM21, and Ligase-dead TRIM21. The GAPDH expression is the loading control. E BRCA1 protein degradation rate in TRIM21^WT and TRIM21^KO cells. Data were shown as mean ± SD. The significant level was identified by *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. One-way ANOVA analysis is used for (D) and (E). F The CO-IP results between TRIM21 and BRCA1. G 293T cells were transfected with Myc-TRIM21, and cell lysates were subjected to analysis by IP, followed by IB detection. H Immunofluorescent staining revealed the colocalization of TRIM21 and BRCA1 proteins in MDA-MB-231 cells. BRCA1 (red) and TRIM21 (green) and nuclear (blue). Bar length: 10 μm. I Colocalization analysis for immunofluorescent stained nuclear by ImageJ with colocalization plugin JACoP. X-axis: red pixel intensity (BRCA1). Y-axis: green pixel intensity (TRIM21). Overlap_R = 0.94, Pearson_R = 0.92 (calculations were based on data from three independent experiments, at least 15 nucleoli were analyzed).