Figure 1.

Umbilical cord occlusion (UCO) model and resuscitation outcomes. A, Timeline of azithromycin (AZM) administration. B, Return of spontaneous circulation (ROSC) was faster in AZM-treated animals, and the time to extubation was shorter compared with the placebo group. Groups were compared using unpaired t test for ROSC assessment and Mann-Whitney U test for extubation time. AZM: n=21, placebo: n=13. C, UCO (time 0 min) induced asystole with corresponding loss in cardiac output and hypotension. ROSC restored blood pressure to levels similar to age-matched controls. Hemodynamic data were analyzed using grouped analysis of the individual group’s means for a specific time point. AZM: n=18; placebo: n=13; control: n=6 (controls data were used with permission from Mike et al²³). D, The incidence of second dose of epinephrine and dextrose administration was similar between the groups. The proportion of variables was assessed using Fisher exact test. AZM: n=21; placebo: n=13. E, There were no differences in selected anthropometric parameters between AZM and placebo. Brain and body weight differences were assessed using ANOVA. AZM: n=21; placebo: n=12; control: n=10. Data shown in the graphs B and E as mean±SEM, graph C are shown as mean, graph D is a contingency table. AZM-treated group, green. Placebo, black. Controls, yellow. CPR indicates cardiopulmonary resuscitation; DBP, diastolic blood pressure; MAP, mean arterial pressure; pK, pharmacokinetic; and SBP, systolic blood pressure. *P<0.05, **P<0.01, ***P<0.001.