Figure 5.

Neurological outcomes. A, We assessed combined outcomes score, as a composite score consisting of motor function and feeds+activity. The summary graphs picture the relationship to uninjured controls. Azithromycin (AZM)-treated group, green. Placebo, black. Controls, yellow (control data were used with permission from Mike et al²³). B, Odds ratios of outcomes measures are reported on a logarithmic scale. The statistical approach is described in the Supplemental Material. Data are presented as mean±SEM. Compared were AZM: n=21, placebo: n=13. P<0.05 was considered significant. C, The correlation matrix pictures Spearman correlation coefficients of selected study parameters with combined neurological outcomes scores on day 6 after the umbilical cord occlusion (UCO) that reached statistical significance (P<0.05). The earliest parameters that correlate with neurological outcomes are time from UCO to return of spontaneous circulation (ROSC), total white blood cell (WBC) counts, and platelets to lymphocytes ratio (PLR) cell ratio on day 1. At a later time point, brain-to-body ratio, the body weight at explant, systemic inflammation response index (SIRI), systemic immune-inflammation index (SII) on day 4, absolute neutrophil count (ANC), neutrophil to lymphocytes ratio (NLR) on day 5, eosinophils (Eos), and platelet (PLT) on day 6, and histological markers of mature oligodendrocytes correlate with neurological outcomes on day 6. D, Lasso analysis of data revealed UCO to ROSC, pH at CPR, and microgliosis in SCWM2 to predict neurological outcomes on day 6. Yellow: P<0.05, n=34. Pre-UCO prior to the umbilical cord occlusion. CC-1 indicates antiadenomatous polyposis coli clone CC-1; CPR, cardiopulmonary resuscitation; Iba-1, ionized calcium-binding adaptor molecule-1; IL-4, interleukin-4; IL-10, interleukin-10; PVWM, periventricular white matter; and SCWM2, subcortical white matter of the first parasagittal gyrus.