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. 2023 Oct 9;8(5):824–834. doi: 10.1089/can.2021.0098

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FIG. 4. — Inhalant CBD decreased ILC frequencies in GBM, regulating local proliferation and activation of ILCs within TME. Flow cytometry analysis showed that inhalant CBD was able to reduce frequencies of ILCs within TME of GBM significantly (p<0.001), potentially improving antitumor immunity. ILCs were characterized by live gating of total tumor cells (a) based on FSC/SSC, and further gating (b) as CD45⁺ lineage negative (CD3e, CD11b, CD24, CD5, CD11c, CD19, NK1.1, Gr-1, TER119, and gd TCR), followed by additional gating (*p > 0.05) (c) as Lin-CD45⁺Thy1⁺. A pie chart (d) displays the representation of the ratio of ILC counts of CBD-treated (green 33%) vs placebo group (red 67%). FSC, forward scatter; ILC, innate lymphoid cell; NK, natural killer; SSC, side scatter. Color images are available online.