Table 1.

Radioligand Binding in HEK293 Cells Stably Expressing Wild-Type or Mutated CB1 Receptor

CB1 receptor	Kd (nM)	Bmax (pmol/mg)
WT	3.48 ± 0.24	29.16 ± 0.62
I2.43A	2.67 ± 0.28	27.32 ± 0.83
I2.43T	3.46 ± 0.75	21.28 ± 1.42a
Y7.53F	4.22 ± 0.42	33.44 ± 1.06
S7.57E	4.88 ± 0.38	53.47 ± 1.41b
N7.49A	4.76 ± 0.40	51.31 ± 1.51b

All data shown as mean ± SEM.

Data from three biological replicates performed in three technical replicates (N = 9) and analyzed by Brown–Forsythe and Welch's ANOVA with Dunnett's post-test: ^ap = 0.049; ^bp < 0.01.

ANOVA, analysis of variance; HEK, human embryonic kidney; SEM, standard error of the mean; WT, wild type.