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. 2023 Oct 11;120(42):e2305950120. doi: 10.1073/pnas.2305950120

Fig. 2.

Fig. 2.

Fiber photometry recordings from MeApd-ERβ+ neurons in response to individually presented social stimuli. (A) Duration of time spent in each contact area (seconds) during RF (red) vs. Empty (gray) preference tests (Mean ± SEM, n = 5). (B) Sniffing duration toward each cylinder (seconds) during RF (red) vs. Empty (gray) preference tests (Mean ± SEM, n = 5). (C) The mean GCaMP7f signals (zFn) during sniffing toward RF (red: 117 events) and Empty (gray: 121 events) cylinders. The data were derived from five male mice and shown for 10 s (2 s before to 8 s after) around the onset of each sniffing event. (D) Mean AUC during 8 s of sniffing behavior, delivered from the positive area of normalized GCaMP7f signals (0 < zFn) in RF (red) vs. Empty (gray) preference tests (Mean ± SEM, n = 5, *P < 0.05). (E) Duration of time spent in each contact area (seconds) during XF (orange) vs. Empty (gray) preference tests (Mean ± SEM, n = 5, *P < 0.05). (F) Sniffing duration toward each cylinder (seconds) during XF (red) vs. Empty (gray) preference tests (Mean ± SEM, n = 5). (G) The mean GCaMP7f signals (zFn) during sniffing toward XF (orange: 131 events) and Empty (gray: 124 events) cylinders. The data were derived from five male mice and shown for 10 s (2 s before to 8 s after) around the onset of each sniffing event. (H) Mean AUC during 8 s of sniffing behavior, delivered from the positive area of normalized GCaMP7f signals (0 < zFn) in XF (orange) vs. Empty (gray) preference tests (Mean ± SEM, n = 5, *P < 0.05). (I) Duration of time spent in each contact area (seconds) during IM (blue) vs. Empty (gray) preference tests (Mean ± SEM, Student’s t-test n = 5, ***P < 0.001). (J) Sniffing duration toward each cylinder (seconds) during IM (blue) vs. Empty (gray) preference tests (Mean ± SEM, n = 5, *P < 0.05). (K) The mean GCaMP7f signals (zFn) during sniffing toward IM (blue: 130 events) and Empty (gray: 114 events) cylinders. The data were derived from five male mice and shown for 10 s (2 s before to 8 s after) around the onset of each sniffing event. (L) Mean AUC during 8 s of sniffing behavior, delivered from the positive area of normalized GCaMP7f signals (0 < zFn) in IM (blue) vs. Empty (gray) preference tests (Mean ± SEM, n = 5, **P < 0.01). MeA, medial amygdala; MeApd, MeA-posterodorsal; ER, estrogen receptor; RF, receptive female; XF, nonreceptive female; IM, intact male; SEM, standard error of the mean.