Table 1.
Antiviral activity of s2U against various RNA viruses (CPE-based assay)
| Virus | Strain | Cell | EC50 (μM)* | Virus | Strain | Cell | EC50 (μM)† |
|---|---|---|---|---|---|---|---|
| MTT/resazurin assay | |||||||
| DENV2 | D2/hu/INDIA/09-74 | BHK-21 | 1.5 ± 0.10 | RABV | HEP | BHK-21 | >100 |
| AG-P10 (Mouse-adapted) | BHK-21 | 1.3 ± 0.12 | LACV | ATCC VR-1834 | MDBK | >100 | |
| ZIKV | MR766 | BHK-21 | 3.8 ± 0.96 | LPHV | 11SB17 | KB | >100 |
| YFV | 17D-204 | BHK-21 | 3.2 ± 1.4 | LCMV | Armstrong | KB | >100 |
| JEV | Beijing-1 | VeroE6 | 7.6 ± 1.3 | JUNV | Candid #1 | 293T | >100 |
| WNV | NY99 | BHK-21 | 8.6 ± 1.8 | SFTSV | ArtLN/2017 | MDCK | >100 |
| VeroE6 | 4.0 ± 0.24 | RVFV | MP12 | MDCK | >100 | ||
| CHIKV | SL10571 | BHK-21 | 3.8 ± 0.12 | TPMV | VRC-66412 | VeroE6 | >100 |
| VeroE6 | 2.2 ± 0.23 | IAV H5N1 | A/Hong Kong/483/97 | A549 | >100 | ||
| HCoV | 229E | MRC5 | 0.94 ± 0.16 | IAV H7N9 | A/Anhui/1/2013 | MA104/TMPRSS2 | >100 |
| HCoV | OC43 | MRC5 | 4.8 ± 0.25 | Virus | Strain | Cell | EC50 (μM)* |
| SARS-CoV-2 | WK-521 (Ancestral) | VeroE6/TMPRSS2 | 2.3 ± 0.43 | SARS-CoV-2 | TY38-873 (Omicron BA.1) | VeroE6/TMPRSS2 | 2.0 ± 0.16 |
| QK002 (Alpha) | VeroE6/TMPRSS2 | 2.2 ± 0.56 | TY40-385 (Omicron BA.2) | VeroE6/TMPRSS2 | 1.8 ± 0.094 | ||
| TY8-612 (Beta) | VeroE6/TMPRSS2 | 2.2 ± 0.23 | TY41-703 (Omicron BA.4) | VeroE6/TMPRSS2 | 1.8 ± 0.013 | ||
| TY7-501 (Gamma) | VeroE6/TMPRSS2 | 1.8 ± 0.37 | TY41-702 (Omicron BA.5) | VeroE6/TMPRSS2 | 2.9 ± 0.18 | ||
| TY11-927 (Delta) | VeroE6/TMPRSS2 | 1.9 ± 0.22 | TY41-796 (Omicron BQ.1.1) | VeroE6/TMPRSS2 | 2.0 ± 0.44 | ||
| MA-P10 (Mouse-adapted) | VeroE6/TMPRSS2 | 2.5 ± 0.34 | TY41-795 (Omicron XBB.1) | VeroE6/TMPRSS2 | 1.9 ± 0.18 | ||
| Virus | Strain | Cell | EC50 (μM)* | Fold change‡ | |||
| Resazurin assay | |||||||
| rgDENV2 | Wild type | BHK-21 | 0.86 ± 0.12 | ||||
| rgDENV2 | NS5-G605V | BHK-21 | 5.1 ± 0.69 | 6.1 | |||
Antiviral assays were carried out as described in SI Appendix, Table S3. EC50: 50% effective concentration.
*EC50 values represent mean values ± SEM from at least three independently performed experiments (n = 2).
‡Fold change is calculated from the ratio of rgNS5-G605V/rgWT.
†EC50 values represent mean values from at least three independently performed experiments (n = 2).