Table 2.
Antiviral activity of s2U against various RNA and DNA viruses (qPCR assay)
| Virus | Strain | Cell | EC50 (μM) | EC90 (μM) | Virus | Strain | Cell | EC50 (μM) | EC90 (μM) |
|---|---|---|---|---|---|---|---|---|---|
| qPCR Assay | |||||||||
| DENV2 | D2/hu/INDIA/09-74 | VeroE6 | 2.4 | 7.4 | SARS-CoV-2 | WK-521 (Ancestral) | VeroE6/TMPRSS2 | 1.0 | 4.3 |
| Huh7 | <0.63 | 3.4 | QK002 (Alpha) | VeroE6/TMPRSS2 | 0.64 | 2.3 | |||
| ZIKV | MR766 | VeroE6 | 3.7 | 23 | TY8-612 (Beta) | VeroE6/TMPRSS2 | 1.7 | 7.6 | |
| YFV | 17D-204 | VeroE6 | <0.63 | 11 | TY7-501 (Gamma) | VeroE6/TMPRSS2 | 1.3 | 3.6 | |
| JEV | Beijing-1 | VeroE6 | 7.5 | 29 | TY11-927 (Delta) | VeroE6/TMPRSS2 | <0.63 | 2.1 | |
| WNV | NY99 | VeroE6 | <0.63 | 10 | TY41-796 (Omicron_BQ.1.1) | VeroE6/TMPRSS2 | 0.74 | 3.6 | |
| CHIKV | SL10571 | VeroE6 | 1.7 | 13 | TY41-795 (Omicron_XBB.1) | VeroE6/TMPRSS2 | <0.63 | 2.7 | |
| HCoV | 229E | MRC5 | 1.3 | 6.2 | RABV | HEP | BHK-21 | >40 | >40 |
| HCoV | OC43 | VeroE6 | 1.1 | 4.2 | RVFV | MP12 | VeroE6 | >40 | >40 |
| SARS-CoV | Hanoi | VeroE6 | 1.2 | 4.5 | HSV-1 | F | VeroE6 | >40 | >40 |
| MERS-CoV | EMC2012 | VeroE6/TMPRSS2 | 2.3 | 8.2 | |||||
Antiviral assays were carried out as described in SI Appendix, Tables S4 and S5. EC50: 50% effective concentration. EC90: 90% effective concentration. EC50 and EC90 values represent mean values from a single experiment with biological triplicates (SI Appendix, Fig. S2).