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. 2023 Oct 12;120(42):e2315591120. doi: 10.1073/pnas.2315591120

Fig. 5.

Fig. 5.

“CYP4A14 deficiency ameliorated MCD-induced hepatic fibrosis. Male wild-type (WT) and cyp4a14−/− mice were fed with an ND (normal diet) or MCD diet for 8 wk. (A) Masson’s staining indicating that MCD-induced hepatic fibrosis was significantly attenuated in cyp4a14−/− mice. (a) WT on ND; (b) WT on MCD; (c) CYP4A14−/− on ND; (d) CYP4A14−/− on MCD. (Magnification: 200×.) (B) CYP4A14 gene deficiency markedly attenuated MCD-induced α-SMA protein expression in the livers as assessed by an immunostaining analysis. n = 5–7. (a) WT on ND; (b) WT on MCD; (c) CYP4A14−/− on ND; (d) CYP4A14−/− on MCD. (Magnification: 200×.) (C) Western blot assay demonstrating reduced protein levels of COL1A2 and αSMA in the livers of cyp4a14−/− mice. n = 3. (D) Quantitative RT-PCR analysis showing reduced mRNA levels of collagen 1a1, collagen 1a2, α-SMA, and TGFβ1 in the livers of cyp4a14−/− mice. mRNA levels of related genes of hepatic fibrosis, n = 5 to 7. *P < 0.05 vs. WT on ND; #P < 0.05 vs. WT on MCD. Data are presented as mean ± SEM.”