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Deoxycholate promotes PI uptake in metabolically active conidia. Conidia were incubated in MEM for 4 h at 37°C and were then treated with deoxycholate (0.5%, 10 min, 23°C). When stained with PI, the number of PI-positive conidia increased by 30% (░⃞) compared to the number of control conidia before culture (□). The PI fluorescence intensity (FL-3) is plotted on the x axis against the number of cells (events) on the y axis. The marker (M1) defines the range of PI-positive conidia.
