FIG. 3.

Survivin overexpression mimics stiffness-mediated ECM protein production in hVSMCs. hVSMCs infected with adenoviruses encoding wild-type (wt) survivin or the GFP control were plated on soft or stiff hydrogels with 10% FBS for 24 h. Total cell lysates were analyzed by immunoblotting for survivin (a), collagen and fibronectin (b), and Lox (c). Levels were normalized to those in GFP-expressing hVSMCs on soft hydrogels. n = 5 (a), n = 3–4 (b), and n = 4 (c). Error bars show SEMs. *p < 0.05; **p < 0.01; and ns, not significant by ANOVA followed by Newman–Keuls post hoc test for multiple comparisons.