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. 2023 Jun 17;260(6):1603–1606. doi: 10.1007/s00709-023-01870-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Differential expression of cannabis-derived PDEVs. A Diagram showing PDEV isolation workflow. B 75–150 leaves of each cultivar were picked and subjected to washing, negative pressure infiltration, and two rounds of high-speed ultracentrifugation. PDEVs were pelleted and quantitated for further use. C PDEVs particle size and concentration was quantitated via particle tracking analysis. D PDEVs were subjected to protein, RNA, and DNA analysis. E PDEVs were subjected to protein analysis via slot-blot analysis. Statistical analysis on obtained data points was performed using one-way analysis of variance (ANOVA) with Tukey post hoc analysis. Statistical significance is indicated by the mean ± standard deviation (SD) and is defined as P ≤ 0.05 ( ∗), P ≤ 0.01 (∗ ∗), and P ≤ 0.001 (∗ ∗ ∗)