TABLE 2.
Photoinactivation of P. gingivalis, A. viscosus, and HCPC-1 cellsa
PS | % Survival for organism or cell line
|
||
---|---|---|---|
P. gingivalis | A. viscosus | HCPC-1 | |
Cationic conjugate | 1.1 ± 0.9 | <0.01 | 102 ± 2.1 |
Anionic conjugate | 23.9 ± 3.3 | 24.3 ± 3.4 | 127 ± 2.9 |
Neutral conjugate | 9.3 ± 2.6 | 21.2 ± 1.5 | 108 ± 4.2 |
Free ce6 | 39.6 ± 2.3 | 52.9 ± 5.9 | 126 ± 2.7 |
CMA | 8.1 ± 1.8 | 2 ± 0.1 | 100 ± 2.5 |
BPD | 81.1 ± 9.4 | 7.6 ± 7.6 | 8.8 ± 0.7 |
Mixture (pL and ce6) | 8.1 ± 1.8 | 2 ± 0.1 | 100 ± 2.5 |
The percent survival of bacteria was assayed by the colony-forming assay following 1 min of treatment with PS and exposure to 15 J/cm2. The viability of HCPC-1 cells was assayed by the microculture tetrazolium assay. Surviving bacteria and epithelial cells were expressed as a percentage of controls receiving PS in the dark out of the incubator. Values for bacteria are the means from three wells, whereas for HCPC-1 cells values are means from six wells, and data are representative of duplicate experiments (± standard errors of the means).