Fig. 2. A granuloma forms around the infected neutrophil swarm.

a–d Mice were infected with 10⁴ CFU WT C. violaceum. a H&E staining of WT livers 1, 3, 5, and 7 dpi. Representative of 10 experiments (3 and 5 dpi) and 5 experiments (1 and 7 dpi), each with 3–4 mice, each with multiple granulomas per section. b Serial sections of WT liver stained by H&E or indicated IHC markers 3, 5, and 7 dpi. Representative of 10 experiments (3 and 5 dpi) and 5 experiments (7 dpi), each with 4 mice, each with multiple granulomas per section. c Serial sections of WT liver stained with indicated IF markers 1, 3, 5, and 7 dpi. Representative of 2 experiments, each with 4 mice, each with multiple granulomas per section. d Visualization of C. violaceum 3 dpi; carrot, coagulative necrotic hepatocytes. Representative of 10 experiments, each with 3–4 mice, each with multiple granulomas per section.