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. 2023 Oct 21;14:303. doi: 10.1186/s13287-023-03534-x

Fig. 3.

Fig. 3

A MSC characterization (phase-contrast microscopy and immunophenotyping of the primary cell culture at Passage 3; cell differentiation into the three lineages (adipo-, osteo-, and chondrogenic); B Bioequivalent characterization (Live/dead staining: live cells—green fluorescence (Calcein AM), dead cells—red fluorescence (propidium iodide), nuclei—blue fluorescence (Hoechst 33,258), laser scanning confocal microscopy; cell viability measured using the AlamarBlue assay; total DNA content measured using the PicoGreen assay)