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. 2023 Sep 1;38(5):813–826. doi: 10.1016/j.virs.2023.08.010

Fig. 7.

Fig. 7

Promoting effect of immunized serum on NADC30-like PRRSV SX-YL1806 replication in vitro. A–E CRL2843-CD163 cells were infected with virus–immunized serums mixture (NADC30-like PRRSV SX-YL1806) for 48 h, then the cells were harvested for the relative quantitative RT-qPCR assay. The MLV-immunized serums were collected from group MLV-SX at 14 dpv, 21 dpv, 28 dpv, 35 dpv, and 42 dpv, respectively; serum collected from group NC was treated as a negative control. Serums were diluted by 1:50, 1:100, 1:200, 1:400, and 1:800. F–G The CRL2843-CD163 cells were infected with the PRRSV-serum complex or PRRSV-serum-Protein A complex for 48 h (NADC30-like PRRSV SX-YL1806), then the cells were harvested for immunofluorescence assay (F) or Western blotting assay (G). The MLV-immunized serums were collected from group MLV-SX at 42 dpv and diluted by 1:50. 2#, 4#, and 5# represented the three piglets in group MLV-SX. The 6D10 was used as the corresponding antibody. Unimmunized pig serums [PRRSV (−)] and PEDV-positive pig [PEDV (+)] serums served as controls. Scale bar ​= ​200 ​μm. Data were shown as mean ​± ​SD ∗P ​< ​0.05; ∗∗P ​< ​0.01; ∗∗∗P < 0.001; ns, not significant.