Table 1.
Overview of various methods for mapping segmental and peripheral distribution of spinal afferent neurons.
|
Retrograde and transganglionic tracing Organ injections |
Rat stomach (HRP): T4-L1 (T8-10) | [139] |
| Mouse dist. colon (fast blue): T8-L1, L6/S1 | [140] | |
| Mouse jejunum (cholera toxin B): T9-T13 | [141] | |
| Mouse dist. colon (cholera toxin B): T9-L1, L4-S2 | [141] | |
| Mouse dist. colon (cholera toxin B): T10-L1, L6/S1 | [45] | |
| Rat liver (HRP): T7 – 11 | [142] | |
| Mouse hepatoportal vein: T8-13 | [107] | |
| Rat pancreas (true blue): T6-L2 (T9-13) | [143] | |
| Mouse pancreas (cholera toxin B): T5-13 (T9-12) | [29] | |
| Hamster iBAT (PRV): T1-3 | [126] | |
| Mouse iBAT (PRV): T2-5 | [62] | |
| Hamster iWAT (fluorogold): T11-L3 | [144] | |
| Mouse iWAT (cholera toxin B): T11-L3 | [145] | |
| Mouse iWAT (PRV): T11-13 | [63] | |
| Rat stomach (HSV-1 H129): T8-9 | [146] | |
|
Retrograde and transganglionic tracing Cut nerve applications |
Cat greater splanchnic nerve (HRP): T7-11 | [147,148] |
| Rat greater splanchnic nerve (HRP): T3-13 (T8-12) | [149] | |
| Rat lumbar splanchnic nerves (HRP): T11-L2 | [40] | |
| Cat lumbar colonic nerves (HRP): T13-L5 (L3-L4) | [150] | |
| Cat hypogastric nerve (HRP): T12-L5 | [151] | |
| Cat pelvic nerve (HRP): S1-3 | [152] | |
| Anterograde tracing from DRG to organs or CNS | C2-4 (WGA-HRP): to rat sternomastoid muscle | [153] |
| T8-13 (CTB-HRP): to cat lower esophageal sphincter | [154,155] | |
| T8-12 (dextran biotin): to mouse upper GIT | [156] | |
| L6-S1 (dextran biotin): to mouse colon | [[157], [158], [159]] | |
| T9-10 (dextran biotin: to WBN/Kob rat pancreas | [160] | |
| T13-L1 (AAV-FP): to mouse iWAT | [145] | |
| T7-10 (HSV-LSL-TK-tomato): to mouse CNS | [50] | |
| Neuronal activity markers | Gastric stimulation | [161] |
| Colorectal distention | [162] | |
| Inflammatory bowel disease | [163] | |
| Fiber photometry | vGlut2-expressing neurons n DRGs 11 and 12 responding to gastric infusion of diverse osmolar stimuli | [24] |