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. 2023 Oct 6;4(10):101201. doi: 10.1016/j.xcrm.2023.101201

Figure 2.

Figure 2

In vitro characterization of SIVdup

(A) Western blot analyses of cells transfected with SIVdup, an env deletion mutant of SIV (Δenv), or wild-type SIV (SIVwt) and virions released from the transfected cells with antibodies to SIV Env and Gag.

(B) Replication kinetics of SIVdup, VSV-G pseudotyped SIVdup, VSV-G pseudotyped Δenv and SIVwt on CEMxSEAP indicator cells exposed to 1 × 104 infectious units of virus. Mean and standard deviation of the secreted embryonic alkaline phosphatase (SEAP) activity of triplicate cultures are shown.

(C) Western blot analyses of cell lysates from day 7 cultures infected as shown in (B) with antibodies to SIV Env, Gag, and actin.

(D) Titers of SIVwt and VSV-G pseudotyped SIVdup in a viral outgrowth assay (TCID50) and a single cycle of infection assay (SCIU). Mean and standard deviation (SD) of three independent experiments and the ratio of the titers are shown. See also Figure S1.