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. 2023 Oct 6;4(10):101201. doi: 10.1016/j.xcrm.2023.101201

Figure 5.

Figure 5

Repeated low-dose challenge of 10–1074 treated rhesus monkeys

(A) Binding of 10–1074 to 293T cells transfected with an empty vector control (Mock) or expression plasmids encoding SIV Env and HIV Env. One representative experiment out of two is shown.

(B) Neutralization of SIVdup pseudotyped with HIV-1 Env (HIV Env) or SIV Env on TZMbl cells by 10–1074. Mean and standard deviation of three replicates of one experiment are shown.

(C) Viral RNA load after repeated low-dose challenge. Rhesus monkeys were intravenously injected with 10 mg/kg body weight of 10–1074 (n = 6) or were mock treated (n = 6) prior to weekly challenges with 6,000 infectious units of HIV Env pseudotyped SIVdup. In monkeys 2706 and 2876 (blue symbols), the repeated challenges were stopped after four inoculations. All other monkeys were challenged until 1 week after the first detection of SIV RNA in the plasma. Plasma viral RNA levels at the indicated time points are shown.

(D) Concentration of 10–1074 in serum at the indicated time points after injection of the antibody. Black symbols indicate the antibody concentrations at the time points of breakthrough infection. Monkey 2865 was exposed to challenge virus 14 times with the last challenge marked by a black arrow.