Table 2.
Blood collection tubes
| Tube | Storage length without contamination | Storage temperature | Collection volume | Study |
|---|---|---|---|---|
| Cell-Free DNA BCT by Streck | 7 days | RT | 10 mL | a stabilizing reagent prevents cell-free DNA contamination by cellular DNA in plasma during blood sample storage and shipping as determined by digital PCR |
| Cell-Free DNA BCT by Streck | 14 days (some contamination) | RT | 10 mL | a stabilizing reagent prevents cell-free DNA contamination by cellular DNA in plasma during blood sample storage and shipping as determined by digital PCR |
| PAXgene Blood ccfDNA by Qiagen | 7 days | RT | 8 mL | performance comparison of blood collection tubes as LB storage system for minimizing cfDNA contamination from genomic DNA |
| Cell-Free DNA Collection Tubes by Roche | 7 days | RT | 8 mL | performance comparison of blood collection tubes as LB storage system for minimizing cfDNA contamination from genomic DNA |
| Cell-Free DNA Collection Tubes by Roche | 14 days (with some contamination) | RT | 8 mL | performance comparison of blood collection tubes as LB storage system for minimizing cfDNA contamination from genomic DNA |
| K2EDTA | 24 h | RT | 7.5 mL | preanalytical blood sample workup for cell-free DNA analysis using droplet digital PCR for future molecular cancer diagnostics |