Figure 1.
Expression level of tRF-3001a is upregulated following diabetic stress
(A) Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays were conducted to compare tRF-3001a expression between non-diabetic retinas and diabetic retinas following 2, 4, or 6 months of STZ injection (n = 6, ∗p < 0.05, Student’s t test).
(B and C) Primary Müller cells, primary retinal ganglion cells (RGCs), retinal endothelial cells (ECs), pericytes, and RPEs were exposed to high glucose (HG, 25 mM) or oxidative stress (H2O2, 100 μM) or were left untreated (Ctrl) for 48 h. The levels of tRF-3001a expression were examined by qRT-PCR assays (n = 4, ∗p < 0.05, Student’s t test).
(D) tRF-3001a expression in fibrovascular membranes of diabetic patients and idiopathic epiretinal membranes of non-diabetic patients was examined by qRT-PCR assays (n = 10, ∗p < 0.05, Student’s t test).
(E) Western blots and quantitative analysis were conducted to assess the levels of ANG and Dicer expression in non-diabetic retinas and diabetic retinas following 2-, 4-, and 6-month diabetes induction (n = 6, ∗p < 0.05, one-way ANOVA followed by post hoc Bonferroni test).
(F and G) Müller cells were transfected with ANG siRNA1-3, Dicer siRNA1-3, or negative control (NC) siRNA for 48 h. qRT-PCR assays were conducted to detect the levels of ANG and Dicer expression (n = 4, ∗p < 0.05, one-way ANOVA followed by post hoc Bonferroni test).
(H and I) Müller cells were transfected with negative control (NC) siRNA, ANG siRNA, or Dicer siRNA, and exposed to high glucose (HG, 25 mM) for 48 h. qRT-PCR assays were conducted to detect the levels of tRF-3001 expression (n = 4, ∗p < 0.05, one-way ANOVA followed by post hoc Bonferroni test). See also Table S1.