Figure 1.

Murine models for inducible expression of human A3B

(A) Schematics of our two different Rosa26 knockin A3B minigene constructs. Human A3B expression at high (CAG-A3B) or low (R26-A3B) levels, respectively, occurs after Cre-mediated excision of the loxP (pink triangle)-flanked transcription stop cassette.

(B and C) Immunoblot and ssDNA deaminase activity of human A3B protein expressed in the indicated tissues from CAG-A3B and R26-A3B animals. Tubulin provides a loading control, and recombinant A3A is a positive control for activity (S, substrate; P, product). Normalized A3B signal quantification relative to tubulin is shown below the immunoblot.

(D) Anti-A3B IHC staining of representative tissues from WT and CAG-A3B mice (40× magnifications are enlargements of regions of the corresponding 10× images).

See also Figure S1.