Figure 3.

CAG-A3B mice exhibit accelerated rates of tumor progression and elevated tumor numbers

(A) Kaplan-Meier curves comparing tumor-free survival of WT (n = 29), R26-A3B (n = 41), and CAG-A3B (n = 14) mice. The number of animals with tumors is shown over the total number of animals in each group (log rank Mantel-Cox test p values indicated). Vertical lines on each curve indicate mice that were censored.

(B) Dot plot of the number of tumors per mouse in each respective genotype (mean ± SEM; Mann-Whitney U test p value indicated).

(C) Pie chart summarizing primary tumor locations in WT and CAG-A3B mice.

(D) Anti-A3B IHC staining of representative tissues from human head and neck squamous cell carcinomas (HNSCCs). Inset boxes show the same tissues at 4× additional magnification.

(E) Anti-A3B IHC staining of representative tissues from CAG-A3B mouse tissues. Inset boxes show portions of the same tissues with 4× additional magnification.

(F) Quantification of anti-A3B IHC staining in HNSCCs (n = 7), CAG-A3B HCCs (n = 5), CAG-A3B lymphomas (n = 7), CAG-A3B healthy liver tissues (n = 4), and CAG-A3B healthy spleens (n = 3) (mean ± SD; Mann-Whitney U test p values indicated).

See also Figures S1–S3 and Tables S1 and S2.