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. 2023 Oct 9;14:1165683. doi: 10.3389/fimmu.2023.1165683

Figure 3.

Figure 3

AM3 treatment of human monocytes skews the differentiation process towards an anti-inflammatory phenotype (A) Microscope images of cells at day 0 and day 6 of the differentiation process, magnification: 20X, scale bar: 100 µM. The areas highlighted in a black rectangle show an area of the same image with a larger magnification to better appreciate the cell morphology. (B) Flow cytometry analysis of macrophage markers at day 6 after exposure to AM3, M-CSF or GM-CSF. (C) Comparisons of MFIs by marker (CD68, CD80, CD86, and CD163) are depicted on the charts. n=9, Friedman test with Dunn’s multiple comparisons. *p<0.05, **p<0.01, ***p<0.001. For (B) and (C) all cells were gated as CD45+ CD206+ live cells.