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. Author manuscript; available in PMC: 2023 Oct 23.
Published in final edited form as: Methods Mol Biol. 2017;1540:179–192. doi: 10.1007/978-1-4939-6700-1_14

Figure 2. Replication inhibition assay.

Figure 2.

Plus-polarity DNA is measured by amplifying HBV DNA across the gap in the minus-polarity DNA strand. Minus-polarity DNA strands are measured by amplifying sequences downstream of the 3’ end of the vast majority of plus-polarity DNA strands in viral capsids.