TABLE 2.
Alterations in GyrA and susceptibilities of fluoroquinolone-resistant clinical isolates of Enterobacteriaceae species
| Strain | MIC (μg/ml) of:
|
Amino acid (codon) change at positiona:
|
||||
|---|---|---|---|---|---|---|
| CIP | OFLX | SPAR | 81 | 83 | 87 | |
| C. freundii | ||||||
| ATCC 8090 | ≤0.12 | ≤0.25 | ≤0.008 | Gly (GGT) | Thr (ACC) | Asp (GAC) |
| 7377 | 2 | 8 | >2 | — | Ile (ATC) | — |
| 0759 | 2 | 8 | >2 | — | Ile (ATC) | — |
| 9085 | 4 | 8 | 2 | — | Ile (ATC) | — |
| 9417 | 8 | 8 | >2 | — | Ile (ATC) | — |
| 1958 | ≥16 | ≥16 | >2 | — | Ile (ATC) | Gly (GGC) |
| 5757 | ≥16 | ≥16 | >2 | — | Ile (ATC) | Gly (GGC) |
| 9023 | ≥16 | ≥16 | >2 | — | Ile (ATC) | — |
| E. aerogenes | ||||||
| ATCC 13048 | 0.5 | 1 | 0.25 | Gly (GGT) | Thr (ACC) | Asp (GAC) |
| 1747 | ≤0.12 | <0.25 | 0.06 | — | — | — |
| 2786 | 2 | 4 | 2 | — | Ile (ATC) | — |
| 9032 | 4 | 4 | 2 | — | Ile (ATC) | — |
| 5593 | 8 | 8 | >2 | — | Ile (ATC) | — |
| 9433 | 8 | ≥16 | >2 | — | Ile (ATC) | — |
| 3521 | 8 | ≥16 | >2 | — | Ile (ATC) | — |
| 5590 | 8 | ≥16 | >2 | — | Ile (ATC) | — |
| 2775 | ≥16 | 32 | >2 | — | Ile (ATC) | — |
| E. cloacae | ||||||
| ATCC 13047 | ≤0.12 | ≤0.25 | 0.03 | Gly (GGT) | Ser (TCC) | Asp (GAC) |
| 1700 | ≤0.12 | <0.25 | 0.06 | — | — | — |
| 1524 | 0.25 | 0.5 | 0.12 | — | — | Asn (AAC) |
| 1963 | 2 | 4 | 2 | — | Phe (TTC) | — |
| 1286 | 2 | 4 | 1 | — | Tyr (TAC) | — |
| 3529 | 4 | 8 | >2 | — | Thr (ACC) | — |
| 1544 | 8 | 8 | >2 | — | Tyr (TAC) | — |
| 1627 | 8 | ≥16 | >2 | — | Tyr (TAC) | — |
| 9028 | ≥16 | 8 | >2 | — | Ile (ATC) | — |
| 1224 | ≥16 | 8 | >2 | — | Phe (TTC) | Asn (AAC) |
| 1251 | ≥16 | ≥16 | >2 | — | Ile (ATC) | — |
| 63 | ≥16 | ≥16 | >2 | — | Phe (TTC) | — |
| 105 | ≥16 | ≥16 | >2 | — | Phe (TTC) | — |
| 1783 | ≥16 | ≥16 | >2 | — | Tyr (TAC) | — |
| 9030 | ≥16 | ≥16 | >2 | — | Tyr (TAC) | — |
| 9031 | ≥16 | >32 | >2 | — | Ile (ATC) | — |
| E. coli | ||||||
| ATCC 11775 | ≤0.12 | ≤0.25 | 0.03 | Gly (GGT) | Ser (TCG) | Asp (GAC) |
| 748 | ≤0.12 | ≤0.25 | 0.03 | — | — | — |
| 3535 | 1 | 2 | 2 | — | Leu (TTG) | — |
| 5524 | 2 | 8 | 2 | — | Leu (TTG) | — |
| 9419 | ≥16 | 32 | >2 | — | Leu (TTG) | Gly (GGA) |
| 9421 | ≥16 | 32 | >2 | — | Leu (TTG) | Tyr (TAC) |
| 9425 | ≥16 | >32 | >2 | — | Leu (TTG) | Asn (AAC) |
| K. pneumoniae | ||||||
| ATCC 13883 | 0.5 | ≤0.25 | 0.06 | Gly (GGC) | Ser (TCC) | Asp (GAC) |
| 570 | ≤0.12 | ≤0.25 | 0.06 | — | — | — |
| 1961 | ≤0.12 | ≤0.25 | 0.03 | — | — | — |
| 1361 | 1 | 2 | 0.5 | — | Phe (TTC) | — |
| 1362 | 1 | 2 | 1 | — | Phe (TTC) | — |
| 1177 | 4 | ≥16 | >2 | — | Phe (TTC) | — |
| 682 | ≥16 | ≥16 | >2 | — | Phe (TTC) | — |
| 1768 | ≥16 | 32 | >2 | — | Tyr (TAC) | — |
| 1775 | ≥16 | >32 | >2 | — | Phe (TTC) | Gly (GGC) |
| 1565 | ≥16 | >32 | >2 | — | Tyr (TAC) | Asn (AAC) |
| K. oxytoca | ||||||
| ATCC 13182 | ≤0.12 | ≤0.25 | 0.12 | Gly (GGT) | Thr (ACT) | Asp (GAC) |
| 702 | ≤0.12 | ≤0.25 | 0.03 | — | — | — |
| 2538 | ≤0.12 | ≤0.25 | 0.06 | — | — | — |
| 57 | 0.5 | 0.05 | 0.25 | — | Ile (ATT) | — |
| 1577 | 4 | 4 | 2 | — | Ile (ATT) | — |
| 1817 | 8 | ≥16 | >2 | — | Ile (ATT) | — |
| 466 | ≥16 | 8 | >2 | — | Ile (ATT) | — |
| 32 | ≥16 | ≥16 | >2 | — | Ile (ATT) | — |
| 1578 | ≥16 | ≥16 | >2 | — | Ile (ATT) | — |
| 1612 | ≥16 | ≥16 | >2 | — | Ile (ATT) | — |
| 2777 | ≥16 | >32 | >2 | — | Ile (ATT) | — |
| P. stuartii | ||||||
| ATCC 29914 | ≤0.12 | ≤0.25 | ≤0.12 | Gly (GGT) | Ser (AGC) | Glu (GAG) |
| 1571 | 0.25 | 1 | 0.5 | — | — | — |
| 1284 | 2 | 4 | 2 | — | Arg (CGC) | — |
| 2469 | 8 | ≥16 | >2 | — | Ile (ATC) | — |
| 2783 | ≥16 | ≥16 | 2 | — | Arg (AGG) | — |
| 9428 | ≥16 | ≥16 | >2 | — | Ile (ATC) | — |
| 7375 | ≥16 | 32 | >2 | — | Ile (ATC) | — |
| 2468 | ≥16 | 32 | >2 | — | Ile (ATC) | — |
| 1773 | ≥16 | >32 | >2 | — | Arg (AGG) | — |
| S. marcescens | ||||||
| ATCC 13880 | 0.5 | 1 | 1 | Gly (GGT) | Ser (AGC) | Asp (GAC) |
| 1714 | 0.25 | 1 | 1 | — | — | — |
| 9745 | 2 | 4 | 2 | — | Ile (ATC) | — |
| 1221 | 4 | 8 | >2 | Cys (TGT) | — | — |
| 1969 | 4 | ≥16 | >2 | — | — | Asn (AAC) |
| 1570 | 8 | 8 | >2 | Cys (TGT) | — | — |
| 5591 | 8 | ≥16 | >2 | — | Arg (AGA) | — |
| 1568 | ≥16 | ≥16 | >2 | — | Arg (CGC) | — |
a
Versus the ATCC type strain sequence. Numbers correspond to the amino acid positions of E. coli GyrA (21). —, no change.