(A) Violin plots showing relative expression of key marker genes in the SG. Horizontal lines indicate median values.
(B) RNAscope in situ staining for genes enriched in SEB-1 (Acp5, Mgst2), and genes enriched in SEB-3 (Awat1, Slc6a19). Arrow, region where gene is highly expressed. Inset, magnified view of Mgst2 staining.
(C) RNAscope staining for Krt79 and Pparg in the SG. Asterisk, RNA-low terminal sebocytes. Left column, untreated wild-type skin. Right column, calcipotriol-treated skin.
(D) Schematic summarizing both direct and indirect paths for differentiation of SG basal cells into sebocytes. Scale bar, 50 μm.