KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-FGFR2 | Cell Signaling | Cat # 23328S |
| Chicken anti-K14 | Biolegend | Cat # 906004 |
| Chicken anti-K5 | Biolegend | Cat # 905903 |
| Rat anti-Ki67 | eBioscience | Cat # 14-5698-80 |
| Goat anti-K79 | Santa Cruz | Cat # sc-243156 |
| Rabbit anti-PPARγ | Cell Signaling | Cat # 2443S |
| Rabbit anti-p44/42 (pErk1/2) | Cell Signaling | Cat # 4370 |
| Chicken anti-GFP | Abcam | Cat # ab13970 |
| Goat anti-Scd1 | Santa Cruz | Cat # sc-14719 |
| Biological samples | ||
| Mouse tissue samples, obtained in accordance with guidelines established by the University of Michigan Unit for Laboratory Animal Medicine | This manuscript | Study protocol # PRO00010041 |
| Chemicals, peptides, and recombinant proteins | ||
| Doxycycline chow (1 g/kg) | BioServ Inc | Cat # F3949 |
| Tamoxifen chow (400 mg/kg, irradiated) | Envigo | Cat # TD.130860 |
| 0.25% Trypsin (no EDTA) | Invitrogen | Cat # 15050065 |
| Albumin, Bovine Fraction V (BSA) | Research Prod. International | Cat # A30075 |
| Hank’s balanced salt solution (HBSS) | Gibco | Cat # 14025092 |
| Nair hair removal lotion | Nair | Cat # B001E6OAM8 |
| Hematoxylin | Sigma | Cat # HHS16 |
| DAPI | Sigma | Cat # 32670 |
| Nile Red | Sigma | Cat # N3013 |
| Pemigatinib (INCB054828) | SelleckChem | Cat # S0088 |
| Calcipotriol (MC903) | Sigma | Cat # C4369 |
| Critical commercial assays | ||
| RNAscope 2.5 HD Reagent Kit-BROWN | ACD (RNAscope) | Cat # 322310 |
| RNAscope 2.5 Pretreat Reagents-H202 and Protease Plus | ACD (RNAscope) | Cat # 322330 |
| RNAscope Target Retrieval | ACD (RNAscope) | Cat # 322000 |
| RNAscope Wash Buffer | ACD (RNAscope) | Cat # 310091 |
| Deposited data | ||
| Data files for single-cell RNA sequencing | This study | GEO: GSE225252 |
| Experimental models: Organisms/strains | ||
| Mouse: Lrig1tm1.1(cre/ERT2)Rjc (Lrig1-CreERT2) | The Jackson Laboratory | Cat # 018418 |
| Mouse: B6.129-Ppargtm2Rev/J (Pparg-flox) | The Jackson Laboratory (by way of Dr. Y. Eugene Chen) | Cat # 004584 |
| Mouse: B6;129-Ppargtm1.1(tTA)/Jmgr/J (AdipoTrak) | The Jackson Laboratory | Cat # 024755 |
| Mouse: B6.Cg-Tg(tetO-cre)1Jaw/J (TRE-Cre) | The Jackson Laboratory (by way of Dr. A. Dlugosz) | Cat # 006234 |
| Mouse: Gt(ROSA)26Sortm1(EYFP)Cos (YFP reporter) | The Jackson Laboratory | Cat # 006148 |
| Mouse: C57BL/6J | The Jackson Laboratory | Cat # 000664 |
| Oligonucleotides | ||
| In situ probe: mouse Acp5 | ACD (RNAscope) | Cat # 465001 |
| In situ probe: mouse Mgst2 | ACD (RNAscope) | Cat # 819931 |
| In situ probe: mouse Awat1 | ACD (RNAscope) | Cat # 1172821-C1 |
| In situ probe: mouse Slc6a19 | ACD (RNAscope) | Cat # 897821 |
| In situ probe: mouse Pparg | ACD (RNAscope) | Cat # 418821 |
| In situ probe: mouse Krt79 | ACD (RNAscope) | Cat # 436201 |
| Software and algorithms | ||
| Cell Ranger v6.1.2 | 10X Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/installation |
| DoubletFinder v2.0 | McGinnis et al.82 | https://github.com/chris-mcginnis-ucsf/DoubletFinder |
| Seurat v4.3.0 | Hao et al.83 | https://github.com/satijalab/seurat |
| COSG v0.9.0 | Dai et al.84 | https://github.com/genecell/COSGR |
| scVelo v0.2.5 | Bergen et al.85 | https://github.com/theislab/scvelo |
| Monocle 2 | Qiu et al.86,87 | https://github.com/cole-trapnell-lab/monocle2-rge-paper |
| clusterProfiler v4.6.0 | Yu et al.88 | https://github.com/YuLab-SMU/clusterProfiler |
| UCell 2.2.0 | Andreatta and Carmona89 | https://github.com/carmonalab/UCell |
| R | R Core | https://www.r-project.org/ |
| Python | Python Software Foundation | https://www.python.org/ |
| org.Mm.eg.db | Carlson90 | http://bioconductor.org/packages/org.Mm.eg.db/ |