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Screening of potential C2 donor substrates for the AprG-catalyzed transaldolation reaction. (A) Structures of possible C2 donors. (B) Extracted ion chromatograms (EICs) at m/z 903.2035 corresponding to [M – H]− for the tridinitrophenyl (DNP)-derivatives of 7, which was obtained after workup of the BtrQ/AprG reactions and then treated with 1-fluoro-2,4-dinitrobenzene (DNFB). Each EIC trace (a–f) is labeled with the compound number of the C2 donor candidate used in the assay.
