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. Author manuscript; available in PMC: 2024 Oct 12.
Published in final edited form as: Cell. 2023 Oct 2;186(21):4528–4545.e18. doi: 10.1016/j.cell.2023.09.009

Figure 2. KDM3B chemical inhibition (KDM3i) promotes transient KMT2A copy gains and break aparts.

Figure 2.

(A) Schematic (top) and quantification of DNA FISH (bottom) demonstrating that KMT2A amplification and break apart events occur with KDM3B inhibitor treatment but no change in copy number at the adjacent CD3 locus in RPE cells.

(B-E) DNA FISH showing that KDM3B inhibition results in KMT2A copy gains and break aparts in KG1a, AML organoids, primary AML cells, and Hematopoietic Stem and Progenitor Cells with no change in copy number at the CD3 locus.

(F) Schematic (top) and quantification of DNA FISH (bottom) showing that KDM3 inhibition (KDM3i) results in KMT2A copy gains and break aparts. Upon KDM3i washout (12hrs Washout), copy gains and break aparts no longer occur. No significant change occurred with the CD3 probe.

Error bars represent the SEM. *p < 0.05 by two-tailed Student’s t-test.