Key resources table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-rhesus IgG1/3 [1B3]-HRP	Nonhuman Primate Reagent Resource (NHPRR)	AB_2819289
ICS and ELISpots	21st Century Biochemicals	M1R, A29L, A35R, and B6R (peptides)
Bacterial and virus strains
mpox (hMPXV/USA/MA001/2022; Lineage B.1, Clade 2b; BEI NR-58622)	Barouch laboratory (this paper)	N/A
MVA:Luc (MVA luciferase reporter virus)	Dr. Mariano Esteban, Centro National de Biotechnologia, Madrid, Spain	N/A
VV:Luc (Vaccinia Western Reserve luciferase reporter virus)	Dr. David Bartlett, University of Pittsburgh, USA	N/A
Biological samples
Chemicals, peptides, and recombinant proteins
Critical commercial assays
U-PLEX Assay Platform (Multiplex ELISA)	Meso Scale Discovery	MSD U-PLEX
NextSeq 500 System WGS Solution (bulk RNA-sequencing)	Illumina, Raczy et al.	NextSeq 500
Deposited data
Raw and analyzed data	This paper	GSE234118
Experimental models: Cell lines
HeLa	ATCC, Manassas, VA	CCL-2
DF-1	Dr. Mark Feinberg, Emory Vaccine Center	N/A
Experimental models: Organisms/strains
rhesus monkeys (Macaca mulatta)	Bioqual, Rockville, MD	N/A
Oligonucleotides
Recombinant DNA
Software and algorithms
R (version 4.1.3)	R Core Team	R.4.1.3
STAR: RNA-seq aligner (version 2.7.10a)	Dobin et al.	STAR
R package: Classification And REgression Training (version 6.0–94)	Kuhn	caret
R package: mixOmics (version 6.24.0)	Lê Cao et al.	mixOmics
R package: Pam: prediction analysis for microarrays (version 1.56.1)	Hastie et al.	pamR
Gene Set Enrichment Analysis (version 4.2.3)	Subramanian et al., Mootha et al.	GSEA_4.2.3
R package: Differential gene expression analysis based on the negative binomial distribution (version 1.40.1)	Love et al.	DESeq2
GraphPad Prism 9.0.0	GraphPad Software	N/A
VersaMax Tunable Microplate Reader	Molecular Devices	N/A
Other